Description
The method employs sandwich ELISA technique. The protein-protein interaction between HRP-RBD and hACE2 can be blocked by neutralizing antibodies against SARS-CoV-2 RBD. Samples and controls are pipetted in a blank microtitre plate and incubated with HRP conjugated human SARSCoV-2 RBD protein. The antibodies to SARS-CoV-2 present in the samples and controls bind to the SARSCoV-2 RBD protein to form a complex. This solution of bound and unbound antibodies to SARS-CoV-2 is then pipetted into human ACE2 coated microplate. After washing to remove the bound complex of Anti-SARS-CoV-2 and HRP conjugated SARSCoV-2 RBD, the substrate solution (TMB) is added to the microwells. Post incubation, color develops proportionally to the amount of unbound Anti-SARS-CoV-2 (Covid-19) present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm